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h1 aavs1 cag gfp  (Addgene inc)
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recombinant pngasef  (Addgene inc)
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dab substrate kit vector labs cat  (Vector Laboratories)
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vectastain abc guinea pig igg kit vector laboratories  (Vector Laboratories)
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1060 5 cytof  (Vector Laboratories)
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vectashield antifade mounting medium  (Vector Laboratories)
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recombinant mil-3  (PeproTech)
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recombinant adenovirus vectors ad5-δe1δe3  (ViraQuest Inc)
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Microtubule Acetylation Is Required for Mechanosensation in Drosophila

doi: 10.1016/j.celrep.2018.09.075

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The donor vector for dTat GFP was generated as follows: pHD-dsRed was digested with EcoRI and the 5′ homology arm corresponding to sequences immediately upstreat of the dTat start codon, GFPand dTat PCR fragments were cloned into the backbone by Gibson assembly (NEB Gibson Assembly Kit) with GFP fused in-frame with the N terminus of dTat and the LoxP-flanked 3×P3-dsRed marker within the second intron.

Techniques: Recombinant